Journal: Cellular and Molecular Gastroenterology and Hepatology
Article Title: Transcriptomic Dissection of Hepatocyte Heterogeneity: Linking Ploidy, Zonation, and Stem/Progenitor Cell Characteristics
doi: 10.1016/j.jcmgh.2019.08.011
Figure Lengend Snippet: Nuclearity-based dissection of 4c hepatocytes. ( A ) Microscopic identification of the nuclearity of 4c hepatocytes using the C1 microfluidics platform. A total of 106 mononucleated and 69 binucleated hepatocytes were included in this analysis. Images were taken using the BZX-710 microscope (Keyence). ( B ) PCA for mononucleated and binucleated 4c hepatocytes using 40 genes. ( C ) Violin plots of nonmonotonically expressed hepatic genes ( top ), zone 3–enriched genes ( middle ), zone 1–enriched genes ( bottom left ), and LPC marker genes ( bottom right ). P values were calculated using the Welch t test.
Article Snippet: Single-cell gene-expression experiments were performed using Fluidigm’s 96.96 or 48.48 quantitative PCR DynamicArray microfluidic chips (Fluidigm) according to the manufacturer’s instructions.
Techniques: Dissection, Microscopy, Marker